Journal: Open Access Rheumatology : Research and Reviews

Article Title: Tofacitinib Inhibits STAT Phosphorylation and Matrix Metalloproteinase-3, -9 and -13 Production by C28/I2 Human Juvenile Chondrocytes

doi: 10.2147/OARRR.S363736

Figure Lengend Snippet: Antibodies Employed for Western Blotting or Immunohistochemistry

Article Snippet: STAT1 , Cell Signaling Technology , 9176 , 1:1000.

Techniques: Western Blot

Journal: Open Access Rheumatology : Research and Reviews

Article Title: Tofacitinib Inhibits STAT Phosphorylation and Matrix Metalloproteinase-3, -9 and -13 Production by C28/I2 Human Juvenile Chondrocytes

doi: 10.2147/OARRR.S363736

Figure Lengend Snippet: Cytokine rhIL-6 induced phosphorylation of STAT1 and STAT3 in C28/I2 chondrocytes and NHC. ( A ) Immunoblot analysis of p-STAT3, total STAT3, and GAPDH in whole cell lysate from C28/I2 cells. ( B ) Immunoblot analysis of p-STAT1, total STAT1, and GAPDH in whole cell lysate from C28/I2 cells. ( C ) Relative abundance of p-STAT3, total STAT3, p-STAT1, and total STAT1 normalized to GAPDH in C28/I2 cells treated as described. ( D ) Immunoblot analysis of p-STAT3, total STAT3, p-STAT1, total STAT1, and GAPDH in whole cell lysate from NHC. ( E ) Relative abundance of p-STAT3, total STAT3, p-STAT1, and total STAT1 normalized to GAPDH in NHC cells treated as described. Immunoblot data is representative of Mean ± Standard Deviation; N=4; *p<0.05, **p<0.01, ***p<0.003.

Article Snippet: STAT1 , Cell Signaling Technology , 9176 , 1:1000.

Techniques: Western Blot, Standard Deviation

Journal: Open Access Rheumatology : Research and Reviews

Article Title: Tofacitinib Inhibits STAT Phosphorylation and Matrix Metalloproteinase-3, -9 and -13 Production by C28/I2 Human Juvenile Chondrocytes

doi: 10.2147/OARRR.S363736

Figure Lengend Snippet: Percent Change in STAT Proteins in Response to IL-6

Article Snippet: STAT1 , Cell Signaling Technology , 9176 , 1:1000.

Techniques:

Journal: Open Access Rheumatology : Research and Reviews

Article Title: Tofacitinib Inhibits STAT Phosphorylation and Matrix Metalloproteinase-3, -9 and -13 Production by C28/I2 Human Juvenile Chondrocytes

doi: 10.2147/OARRR.S363736

Figure Lengend Snippet: Effect of Tofacitinib on rhIL-6 induced p-STAT3 and p-STAT1 in NHC and C28/I2. NHC and C28/I2 were pre-incubated with Tofacitinib (2.5nM, 20nM, 50nM, or 100 nM) for 30 min and then treated with rhIL-6 (20ng/mL) and Tofacitinib (2.5nM, 20nM, 50nM, or 100nM) concomitantly for 30 min. ( A ) Immunoblot analysis of p-STAT3, total STAT3, p-STAT1, total STAT1, and GAPDH in whole cell lysate from NHC or C28/I2 cells. ( B ) Relative abundance of p-STAT1 or p-STAT3 normalized to GAPDH in NHC or C28/I2 cells treated as described. Immunoblot data is representative of Mean ± Standard Deviation; N=4; *p<0.05, **p<0.01, ***p<0.002.

Article Snippet: STAT1 , Cell Signaling Technology , 9176 , 1:1000.

Techniques: Incubation, Western Blot, Standard Deviation

Journal: Oncology reports

Article Title: SAHA down-regulates the expression of indoleamine 2,3-dioxygenase via inhibition of the JAK/STAT1 signaling pathway in gallbladder carcinoma cells.

doi: 10.3892/or.2012.2073

Figure Lengend Snippet: Figure 3. SAHA inhibits STAT1 phosphorylation and nuclear translocation. (A) Cells were treated with or without 10 µM SAHA for 2 h and then treated with 500 U/ml IFN-γ for 5, 10 or 30 min. The phosphorylation of STAT1 and total STAT1 were detected by Western blotting with an anti-pSTAT1-Tyr701 antibody and an anti-STAT1 antibody, respectively. β-actin served as the loading control. Similar results were obtained in three independent experiments. (B) Cells were grown on chamber slides and were pre-treated with or without SAHA (10 µM) for 2 h, followed by treatment with or without IFN-γ (500 U/ml) for 30 min. Immunofluorescence and confocal microscopy were performed as described in Materials and methods. Scale bar, 20 µm.

Article Snippet: Monoclonal antiSTAT1 (9H2) and anti-phospho-Stat1 (Tyr701) antibodies were acquired from a commercial source (Cell Signaling Technology, Beverly, MA).

Techniques: Phospho-proteomics, Translocation Assay, Western Blot, Control, Immunofluorescence, Confocal Microscopy